Determinants and Expansion of Specificity in a Trichothecene UDP-Glucosyltransferase from Oryza sativa.
Identifieur interne : 000C86 ( Main/Exploration ); précédent : 000C85; suivant : 000C87Determinants and Expansion of Specificity in a Trichothecene UDP-Glucosyltransferase from Oryza sativa.
Auteurs : Karl M. Wetterhorn [États-Unis] ; Kaitlyn Gabardi [États-Unis] ; Herbert Michlmayr [Autriche] ; Alexandra Malachova [Autriche] ; Mark Busman [États-Unis] ; Susan P. Mccormick [États-Unis] ; Franz Berthiller [Autriche] ; Gerhard Adam [Autriche] ; Ivan Rayment [États-Unis]Source :
- Biochemistry [ 1520-4995 ] ; 2017.
Descripteurs français
- KwdFr :
- Cinétique (MeSH), Fusarium (métabolisme), Glucosides (MeSH), Glucosyltransferases (composition chimique), Glucosyltransferases (métabolisme), Glycogen debranching enzyme system (MeSH), Hordeum (enzymologie), Maladies des plantes (MeSH), Mutagenèse dirigée (MeSH), Mycotoxines (métabolisme), Oryza (enzymologie), Oryza (métabolisme), Protéines végétales (métabolisme), Trichothécènes (composition chimique), Triticum (MeSH).
- MESH :
- composition chimique : Glucosyltransferases, Trichothécènes.
- enzymologie : Hordeum, Oryza.
- métabolisme : Fusarium, Glucosyltransferases, Mycotoxines, Oryza, Protéines végétales.
- Cinétique, Glucosides, Glycogen debranching enzyme system, Maladies des plantes, Mutagenèse dirigée, Triticum.
English descriptors
- KwdEn :
- Fusarium (metabolism), Glucosides (MeSH), Glucosyltransferases (chemistry), Glucosyltransferases (metabolism), Glycogen Debranching Enzyme System (MeSH), Hordeum (enzymology), Kinetics (MeSH), Mutagenesis, Site-Directed (MeSH), Mycotoxins (metabolism), Oryza (enzymology), Oryza (metabolism), Plant Diseases (MeSH), Plant Proteins (metabolism), Trichothecenes (chemistry), Triticum (MeSH).
- MESH :
- chemical , chemistry : Glucosyltransferases, Trichothecenes.
- chemical , metabolism : Glucosyltransferases, Mycotoxins, Plant Proteins.
- chemical : Glucosides, Glycogen Debranching Enzyme System.
- enzymology : Hordeum, Oryza.
- metabolism : Fusarium, Oryza.
- Kinetics, Mutagenesis, Site-Directed, Plant Diseases, Triticum.
Abstract
Family 1 UDP-glycosyltransferases (UGTs) in plants primarily form glucose conjugates of small molecules and, besides other functions, play a role in detoxification of xenobiotics. Indeed, overexpression of a barley UGT in wheat has been shown to control Fusarium head blight, which is a plant disease of global significance that leads to reduced crop yields and contamination with trichothecene mycotoxins such as deoxynivalenol (DON), T-2 toxin, and many other structural variants. The UGT Os79 from rice has emerged as a promising candidate for inactivation of mycotoxins because of its ability to glycosylate DON, nivalenol, and hydrolyzed T-2 toxin (HT-2). However, Os79 is unable to modify T-2 toxin (T-2), produced by pathogens such as Fusarium sporotrichioides and Fusarium langsethii. Activity toward T-2 is desirable because it would allow a single UGT to inactivate co-occurring mycotoxins. Here, the structure of Os79 in complex with the products UDP and deoxynivalenol 3-O-glucoside is reported together with a kinetic analysis of a broad range of trichothecene mycotoxins. Residues associated with the trichothecene binding pocket were examined by site-directed mutagenesis that revealed that trichothecenes substituted at the C4 position, which are not glycosylated by wild-type Os79, can be accommodated in the binding pocket by increasing its volume. The H122A/L123A/Q202L triple mutation, which increases the volume of the active site and attenuates polar contacts, led to strong and equivalent activity toward trichothecenes with C4 acetyl groups. This mutant enzyme provides the broad specificity required to control multiple toxins produced by different Fusarium species and chemotypes.
DOI: 10.1021/acs.biochem.7b01007
PubMed: 29140092
Affiliations:
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Wetterhorn</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706</wicri:regionArea><wicri:noRegion>Wisconsin 53706</wicri:noRegion></affiliation></author><author><name sortKey="Gabardi, Kaitlyn" sort="Gabardi, Kaitlyn" uniqKey="Gabardi K" first="Kaitlyn" last="Gabardi">Kaitlyn Gabardi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706</wicri:regionArea><wicri:noRegion>Wisconsin 53706</wicri:noRegion></affiliation></author><author><name sortKey="Michlmayr, Herbert" sort="Michlmayr, Herbert" uniqKey="Michlmayr H" first="Herbert" last="Michlmayr">Herbert Michlmayr</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU) , Konrad-Lorenz-Strasse 24, 3430 Tulln, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU) , Konrad-Lorenz-Strasse 24, 3430 Tulln</wicri:regionArea><wicri:noRegion>3430 Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Malachova, Alexandra" sort="Malachova, Alexandra" uniqKey="Malachova A" first="Alexandra" last="Malachova">Alexandra Malachova</name><affiliation wicri:level="1"><nlm:affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism, Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU) , Konrad-Lorenz-Strasse 20, 3430 Tulln, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Christian Doppler Laboratory for Mycotoxin Metabolism, Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU) , Konrad-Lorenz-Strasse 20, 3430 Tulln</wicri:regionArea><wicri:noRegion>3430 Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Busman, Mark" sort="Busman, Mark" uniqKey="Busman M" first="Mark" last="Busman">Mark Busman</name><affiliation wicri:level="1"><nlm:affiliation>Mycotoxin Prevention and Applied Microbiology Research Unit, USDA/ARS, National Center for Agricultural Utilization Research , Peoria, Illinois 61604, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mycotoxin Prevention and Applied Microbiology Research Unit, USDA/ARS, National Center for Agricultural Utilization Research , Peoria, Illinois 61604</wicri:regionArea><wicri:noRegion>Illinois 61604</wicri:noRegion></affiliation></author><author><name sortKey="Mccormick, Susan P" sort="Mccormick, Susan P" uniqKey="Mccormick S" first="Susan P" last="Mccormick">Susan P. Mccormick</name><affiliation wicri:level="1"><nlm:affiliation>Mycotoxin Prevention and Applied Microbiology Research Unit, USDA/ARS, National Center for Agricultural Utilization Research , Peoria, Illinois 61604, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mycotoxin Prevention and Applied Microbiology Research Unit, USDA/ARS, National Center for Agricultural Utilization Research , Peoria, Illinois 61604</wicri:regionArea><wicri:noRegion>Illinois 61604</wicri:noRegion></affiliation></author><author><name sortKey="Berthiller, Franz" sort="Berthiller, Franz" uniqKey="Berthiller F" first="Franz" last="Berthiller">Franz Berthiller</name><affiliation wicri:level="1"><nlm:affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism, Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU) , Konrad-Lorenz-Strasse 20, 3430 Tulln, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Christian Doppler Laboratory for Mycotoxin Metabolism, Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU) , Konrad-Lorenz-Strasse 20, 3430 Tulln</wicri:regionArea><wicri:noRegion>3430 Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Adam, Gerhard" sort="Adam, Gerhard" uniqKey="Adam G" first="Gerhard" last="Adam">Gerhard Adam</name><affiliation wicri:level="1"><nlm:affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU) , Konrad-Lorenz-Strasse 24, 3430 Tulln, Austria.</nlm:affiliation><country xml:lang="fr">Autriche</country><wicri:regionArea>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU) , Konrad-Lorenz-Strasse 24, 3430 Tulln</wicri:regionArea><wicri:noRegion>3430 Tulln</wicri:noRegion></affiliation></author><author><name sortKey="Rayment, Ivan" sort="Rayment, Ivan" uniqKey="Rayment I" first="Ivan" last="Rayment">Ivan Rayment</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706</wicri:regionArea><wicri:noRegion>Wisconsin 53706</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biochemistry</title><idno type="eISSN">1520-4995</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fusarium (metabolism)</term><term>Glucosides (MeSH)</term><term>Glucosyltransferases (chemistry)</term><term>Glucosyltransferases (metabolism)</term><term>Glycogen Debranching Enzyme System (MeSH)</term><term>Hordeum (enzymology)</term><term>Kinetics (MeSH)</term><term>Mutagenesis, Site-Directed (MeSH)</term><term>Mycotoxins (metabolism)</term><term>Oryza (enzymology)</term><term>Oryza (metabolism)</term><term>Plant Diseases (MeSH)</term><term>Plant Proteins (metabolism)</term><term>Trichothecenes (chemistry)</term><term>Triticum (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cinétique (MeSH)</term><term>Fusarium (métabolisme)</term><term>Glucosides (MeSH)</term><term>Glucosyltransferases (composition chimique)</term><term>Glucosyltransferases (métabolisme)</term><term>Glycogen debranching enzyme system (MeSH)</term><term>Hordeum (enzymologie)</term><term>Maladies des plantes (MeSH)</term><term>Mutagenèse dirigée (MeSH)</term><term>Mycotoxines (métabolisme)</term><term>Oryza (enzymologie)</term><term>Oryza (métabolisme)</term><term>Protéines végétales (métabolisme)</term><term>Trichothécènes (composition chimique)</term><term>Triticum (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Glucosyltransferases</term><term>Trichothecenes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucosyltransferases</term><term>Mycotoxins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Glucosides</term><term>Glycogen Debranching Enzyme System</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Glucosyltransferases</term><term>Trichothécènes</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Hordeum</term><term>Oryza</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Hordeum</term><term>Oryza</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fusarium</term><term>Oryza</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fusarium</term><term>Glucosyltransferases</term><term>Mycotoxines</term><term>Oryza</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Kinetics</term><term>Mutagenesis, Site-Directed</term><term>Plant Diseases</term><term>Triticum</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cinétique</term><term>Glucosides</term><term>Glycogen debranching enzyme system</term><term>Maladies des plantes</term><term>Mutagenèse dirigée</term><term>Triticum</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Family 1 UDP-glycosyltransferases (UGTs) in plants primarily form glucose conjugates of small molecules and, besides other functions, play a role in detoxification of xenobiotics. Indeed, overexpression of a barley UGT in wheat has been shown to control Fusarium head blight, which is a plant disease of global significance that leads to reduced crop yields and contamination with trichothecene mycotoxins such as deoxynivalenol (DON), T-2 toxin, and many other structural variants. The UGT Os79 from rice has emerged as a promising candidate for inactivation of mycotoxins because of its ability to glycosylate DON, nivalenol, and hydrolyzed T-2 toxin (HT-2). However, Os79 is unable to modify T-2 toxin (T-2), produced by pathogens such as Fusarium sporotrichioides and Fusarium langsethii. Activity toward T-2 is desirable because it would allow a single UGT to inactivate co-occurring mycotoxins. Here, the structure of Os79 in complex with the products UDP and deoxynivalenol 3-O-glucoside is reported together with a kinetic analysis of a broad range of trichothecene mycotoxins. Residues associated with the trichothecene binding pocket were examined by site-directed mutagenesis that revealed that trichothecenes substituted at the C4 position, which are not glycosylated by wild-type Os79, can be accommodated in the binding pocket by increasing its volume. The H122A/L123A/Q202L triple mutation, which increases the volume of the active site and attenuates polar contacts, led to strong and equivalent activity toward trichothecenes with C4 acetyl groups. This mutant enzyme provides the broad specificity required to control multiple toxins produced by different Fusarium species and chemotypes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29140092</PMID><DateCompleted><Year>2017</Year><Month>12</Month><Day>27</Day></DateCompleted><DateRevised><Year>2017</Year><Month>12</Month><Day>27</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-4995</ISSN><JournalIssue CitedMedium="Internet"><Volume>56</Volume><Issue>50</Issue><PubDate><Year>2017</Year><Month>Dec</Month><Day>19</Day></PubDate></JournalIssue><Title>Biochemistry</Title><ISOAbbreviation>Biochemistry</ISOAbbreviation></Journal><ArticleTitle>Determinants and Expansion of Specificity in a Trichothecene UDP-Glucosyltransferase from Oryza sativa.</ArticleTitle><Pagination><MedlinePgn>6585-6596</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.biochem.7b01007</ELocationID><Abstract><AbstractText>Family 1 UDP-glycosyltransferases (UGTs) in plants primarily form glucose conjugates of small molecules and, besides other functions, play a role in detoxification of xenobiotics. Indeed, overexpression of a barley UGT in wheat has been shown to control Fusarium head blight, which is a plant disease of global significance that leads to reduced crop yields and contamination with trichothecene mycotoxins such as deoxynivalenol (DON), T-2 toxin, and many other structural variants. The UGT Os79 from rice has emerged as a promising candidate for inactivation of mycotoxins because of its ability to glycosylate DON, nivalenol, and hydrolyzed T-2 toxin (HT-2). However, Os79 is unable to modify T-2 toxin (T-2), produced by pathogens such as Fusarium sporotrichioides and Fusarium langsethii. Activity toward T-2 is desirable because it would allow a single UGT to inactivate co-occurring mycotoxins. Here, the structure of Os79 in complex with the products UDP and deoxynivalenol 3-O-glucoside is reported together with a kinetic analysis of a broad range of trichothecene mycotoxins. Residues associated with the trichothecene binding pocket were examined by site-directed mutagenesis that revealed that trichothecenes substituted at the C4 position, which are not glycosylated by wild-type Os79, can be accommodated in the binding pocket by increasing its volume. The H122A/L123A/Q202L triple mutation, which increases the volume of the active site and attenuates polar contacts, led to strong and equivalent activity toward trichothecenes with C4 acetyl groups. This mutant enzyme provides the broad specificity required to control multiple toxins produced by different Fusarium species and chemotypes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wetterhorn</LastName><ForeName>Karl M</ForeName><Initials>KM</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gabardi</LastName><ForeName>Kaitlyn</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Michlmayr</LastName><ForeName>Herbert</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU) , Konrad-Lorenz-Strasse 24, 3430 Tulln, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Malachova</LastName><ForeName>Alexandra</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism, Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU) , Konrad-Lorenz-Strasse 20, 3430 Tulln, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Busman</LastName><ForeName>Mark</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Mycotoxin Prevention and Applied Microbiology Research Unit, USDA/ARS, National Center for Agricultural Utilization Research , Peoria, Illinois 61604, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McCormick</LastName><ForeName>Susan P</ForeName><Initials>SP</Initials><AffiliationInfo><Affiliation>Mycotoxin Prevention and Applied Microbiology Research Unit, USDA/ARS, National Center for Agricultural Utilization Research , Peoria, Illinois 61604, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Berthiller</LastName><ForeName>Franz</ForeName><Initials>F</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-5883-2879</Identifier><AffiliationInfo><Affiliation>Christian Doppler Laboratory for Mycotoxin Metabolism, Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU) , Konrad-Lorenz-Strasse 20, 3430 Tulln, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Adam</LastName><ForeName>Gerhard</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU) , Konrad-Lorenz-Strasse 24, 3430 Tulln, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rayment</LastName><ForeName>Ivan</ForeName><Initials>I</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-9279-7835</Identifier><AffiliationInfo><Affiliation>Department of Biochemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>11</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochemistry</MedlineTA><NlmUniqueID>0370623</NlmUniqueID><ISSNLinking>0006-2960</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005960">Glucosides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006004">Glycogen Debranching Enzyme System</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009183">Mycotoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014255">Trichothecenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C542050">deoxynivalenol-3-glucoside</NameOfSubstance></Chemical><Chemical><RegistryNumber>5WOP02RM1U</RegistryNumber><NameOfSubstance UI="C038405">nivalenol</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="D005964">Glucosyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.25</RegistryNumber><NameOfSubstance UI="C020569">4 alpha-glucanotransferase</NameOfSubstance></Chemical><Chemical><RegistryNumber>JT37HYP23V</RegistryNumber><NameOfSubstance UI="C007262">deoxynivalenol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005670" MajorTopicYN="N">Fusarium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005960" MajorTopicYN="N">Glucosides</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005964" MajorTopicYN="N">Glucosyltransferases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006004" MajorTopicYN="N">Glycogen Debranching Enzyme System</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001467" MajorTopicYN="N">Hordeum</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016297" MajorTopicYN="N">Mutagenesis, Site-Directed</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009183" MajorTopicYN="N">Mycotoxins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012275" MajorTopicYN="N">Oryza</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014255" MajorTopicYN="N">Trichothecenes</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>11</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>12</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>11</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29140092</ArticleId><ArticleId IdType="doi">10.1021/acs.biochem.7b01007</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Autriche</li><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Wetterhorn, Karl M" sort="Wetterhorn, Karl M" uniqKey="Wetterhorn K" first="Karl M" last="Wetterhorn">Karl M. Wetterhorn</name></noRegion><name sortKey="Busman, Mark" sort="Busman, Mark" uniqKey="Busman M" first="Mark" last="Busman">Mark Busman</name><name sortKey="Gabardi, Kaitlyn" sort="Gabardi, Kaitlyn" uniqKey="Gabardi K" first="Kaitlyn" last="Gabardi">Kaitlyn Gabardi</name><name sortKey="Mccormick, Susan P" sort="Mccormick, Susan P" uniqKey="Mccormick S" first="Susan P" last="Mccormick">Susan P. Mccormick</name><name sortKey="Rayment, Ivan" sort="Rayment, Ivan" uniqKey="Rayment I" first="Ivan" last="Rayment">Ivan Rayment</name></country><country name="Autriche"><noRegion><name sortKey="Michlmayr, Herbert" sort="Michlmayr, Herbert" uniqKey="Michlmayr H" first="Herbert" last="Michlmayr">Herbert Michlmayr</name></noRegion><name sortKey="Adam, Gerhard" sort="Adam, Gerhard" uniqKey="Adam G" first="Gerhard" last="Adam">Gerhard Adam</name><name sortKey="Berthiller, Franz" sort="Berthiller, Franz" uniqKey="Berthiller F" first="Franz" last="Berthiller">Franz Berthiller</name><name sortKey="Malachova, Alexandra" sort="Malachova, Alexandra" uniqKey="Malachova A" first="Alexandra" last="Malachova">Alexandra Malachova</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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